Coding
Part:BBa_K2963023
Designed by: Haixin Gao Group: iGEM19_JNU-China (2019-09-12)
BsracE200 - Bicistronic containing RBS200 to stabilize gene expression.
Usage and Biology
The bicistronic has a translational coupling effect. Using bicistronics can stabilize the expression of the target gene.In order to more precisely regulate the activity of the racE gene, our team worked with the master in our lab to further stabilize the expression of the racE gene by using the bicistronic system.
Reference
Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, Corynebacterium glutamicum." Engineering in Life Sciences (2017).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 996
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |